Growth Inhibition in Thiobacillus neapolitanus by Histidine, Methionine, Phenylalanine, and Threonine

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Growth Inhibition in Thiobacillus neapolitanus by Histidine, Methionine, Phenylalanine, and Threonine.

Thiobacillus neapolitanus, a strict chemoautotroph, is sensitive to the addition of 10(-4)m methionine, histidine, threonine, or phenylalanine to the thiosulfate medium on which it grows. When histidine, threonine, or phenylalanine are added at the time of inoculation, spontaneous mutants tolerant to the three amino acids are selected. These mutants appear to result from a single genetic change...

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Assimilation and metabolism of exogenous organic compounds by the strict autotrophs Thiobacillus thioparus and Thiobacillus neapolitanus.

The assimilation and utilization of the individual carbon atoms of pyruvate and acetate by cells of Thiobacillus thioparus and T. neapolitanus, in the presence and absence of an energy source, were studied by use of radioactive substrates. Both organisms produced (14)CO(2) from (14)C-labeled pyruvate, but more came from carbon 1 than from carbons 2 or 3. The conversion of the carbons of acetate...

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Stabilities of vanadyl complexes with methionine, phenylalanine, and threonine.

Stepwise formation constants corresponding to 1:1 vanadyl complexes with methionine, phenyl­ alanine and threonine have been determined at 25 °C, and at various ionic concentrations, viz. 0.01, 0.1 and 0.3 M, maintained by the addition of potassium chloride solution. Thermodynamic stability constants have been obtained by extrapolation of log K values to zero ionic strength. Logarithms of the o...

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Properties of phosphoribulokinase from Thiobacillus neapolitanus.

Partially purified preparations of ribulose-5-phosphate kinase (specific activity, 50 to 125 mumoles per min per mg of protein) were employed in a series of kinetic experiments in the presence of several concentrations of H(+), Mg(2+), adenosine triphosphate (ATP), and phosphoenolpyruvate (PEP). The pH optimum of the enzyme was found to be 7.9; at this pH and above, response of the enzyme to va...

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Reduced nicotinamide adenine dinucleotide oxidation by Thiobacillus neapolitanus and Thiobacillus strain C.

A. J. Smith, J. London, and R. Y. Stanier (J. Bacteriol. 94:972, 1967) reported that a number of obligate autotrophs, including Thiobacillus thiooxidans and T. thioparus, lacked reduced nicotinamide adenine dinucleotide (NADH2) oxidase activity, although this activity was readily detected in the facultative autotrophs T. intermedius and Hydrogenomonas eutropha. They suggested that the absence o...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1970

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.104.3.1145-1150.1970